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10x Objectives Comparison

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lrbguy's Avatar
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 Posted 04/13/2016  5:45 pm  Show Profile   Bookmark this reply Add lrbguy to your friends list Get a Link to this Reply
Looking at your setup, austrokiwi, I thought this looked like a pretty good item.

http://www.ebay.com/itm/371579679057

It allows movement in XYZ planes. However, I don't think that there are micrometer rulings. Should I look for some kind of reticule to mount on the X and Y movement rails?

Add one of these?
http://www.ebay.com/itm/181606261433


My big question for both of you (austrokiwi and rmpsrpms) is this, what is the critical direction of movement of the coin with respect to the lens when one is creating the photo stack? (May I assume the direction of shooting is straight down the Z axis)?

Ray, is the stack created from shots that vary in depth?


Edit: here's another one for about the same price -
http://www.ebay.com/itm/322068713895
Edited by lrbguy
04/13/2016 6:00 pm
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 Posted 04/13/2016  6:08 pm  Show Profile   Bookmark this reply Add rmpsrpms to your friends list Get a Link to this Reply
You're correct, the critical direction is vertical, assuming the camera is looking straight down on the coin. The stack is created by varying the distance between camera/lens and coin.

As long as you are not planning to go to higher magnification than 10x, I see no reason to go with a stripped-down microscope stand. The main reason to use one is to get higher vertical resolution, but even at 10x you generally don't need better than 10um resolution, and this can be achieved with the smaller and cheaper Z-stage I linked to. Plus, it requires no modifications be made!

But if you do go the stand route, make sure you get one that has the correct adjustments. You are looking for a stand that has concentric coarse/fine adjustment knobs. These have "infinite" fine step capability, ie you can turn the fine focus knob forever and it will keep moving the stage. Some don't have fine focus at all, while others have limited range to the fine focus. I don't think the Leitz stands you linked to have this infinite capability, but I may be wrong. Not my area of expertise.
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pepactonius's Avatar
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 Posted 04/13/2016  6:13 pm  Show Profile   Bookmark this reply Add pepactonius to your friends list Get a Link to this Reply

Quote:
Should I look for some kind of reticule to mount on the X and Y movement rails?


The Zeiss microscope stage I have has measurement reticles in the X and Y directions, but I never really look at them. Of course, the Z-axis markings on the fine focus knob are useful when stacking.
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 Posted 04/13/2016  6:37 pm  Show Profile   Bookmark this reply Add rmpsrpms to your friends list Get a Link to this Reply
You usually only use XY adjustments to frame the shot, so precise measurement is not needed. I suppose if you do stitching then it's important to have reasonable XY measurements but even then you can probably do it visually since you will almost certainly ensure some degree of overlap.
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 Posted 04/13/2016  11:32 pm  Show Profile   Bookmark this reply Add austrokiwi to your friends list Get a Link to this Reply
RMPSRMPS has answered your question but I would add the following:
When stacking using Microscope objectives I use the Commercially sold Cognisys focusing rail. I purchased the stack-shot system before I learned that a microscope could do the same job. The microscope I use has the fine focusing system that rmpsrmps mentioned its a CH2. I know that some people actually build their own stacking system by motorizing the fine focus using a stepper motor. I use the CH2 as a focus stage for most non stacked coin shots.
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